This study developed a recombinant snow flea antifreeze peptide (rsfAFP) expression system. rsfAFP showed ice recrystallization inhibition, thermal hysteresis activity, and altered ice nucleation, improving the survival rate, acid-producing ability, freezing stability, and cellular metabolism activity of *Streptococcus thermophilus*. rsfAFP interacts with the membrane and ice crystals, forming a protective layer. This research provides a scientific basis for using rsfAFP as an effective antifreeze agent for lactic acid bacteria cryopreservation or other frozen food.
Publisher
npj Science of Food
Published On
Feb 03, 2022
Authors
Xu Chen, Jinhong Wu, Xiaozhen Li, Fujia Yang, Dan Huang, Jianlian Huang, Shaoyun Wang, Vincent Guyonnet
Tags
antifreeze peptide
rsfAFP
Streptococcus thermophilus
cryopreservation
frozen food
metabolism
ice recrystallization
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