Medicine and Health

ANCA: artificial nucleic acid circuit with Argonaute protein for one-step isothermal detection of antibiotic-resistant bacteria

H. Jang, J. Song, et al.

Discover ANCA, a groundbreaking artificial nucleic acid circuit developed by a team of researchers led by Hyowon Jang and Jayeon Song, which enables rapid, amplification-free detection of antibiotic-resistant bacteria like CPKP. This innovative method promises 100% sensitivity and specificity, potentially transforming diagnostics and preventing nosocomial infections.

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Abstract

This paper introduces ANCA, an artificial nucleic acid circuit utilizing Argonaute (Ago) proteins for one-step, amplification-free, and isothermal detection of antibiotic-resistant bacteria. The ANCA method employs a continuous autocatalytic reaction via Ago protein cross-catalytic cleavage. Successful detection of carbapenemase-producing Klebsiella pneumoniae (CPKP) in various samples (urine, blood, and swabs) is demonstrated, achieving 100% sensitivity and specificity in renal wash specimens. The method shows promise for rapid and accurate CPKP diagnosis, aiding in preventing nosocomial infections.

Publisher

Nature Communications

Published On

Dec 05, 2023

Authors

Hyowon Jang, Jayeon Song, Sunjoo Kim, Jung-Hyun Byun, Kyoung G Lee, Kwang-Hyun Park, Euijeon Woo, Eun-Kyung Lim, Juyeon Jung, Taejoon Kang

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