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Single-cell T-cell receptor repertoire profiling in dogs

Veterinary Science

Single-cell T-cell receptor repertoire profiling in dogs

M. H. Hoang, Z. L. Skidmore, et al.

Discover how single-cell T-cell receptor sequencing (scTCRseq) provides insights into immune responses in dogs with cancer. This groundbreaking research, led by My H. Hoang and colleagues, reveals the diverse T-cell repertoires in healthy dogs and distinct profiles in those with T-cell lymphoma and melanoma, highlighting potential anti-tumor activity.

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Playback language: English
Abstract
This study demonstrates the applicability of single-cell T-cell receptor sequencing (scTCRseq) in dogs with cancer. Researchers profiled PBMCs or lymph node aspirates from healthy dogs, dogs with T-cell lymphoma, and dogs with melanoma using scRNA and dog-specific scTCRseq primers. Healthy dogs showed highly diverse repertoires, lymphomas exhibited clonal repertoires, and melanoma dogs displayed a limited number of abundant clonotypes. Analysis of dominant clonotypes in melanoma PBMCs revealed predominantly activated CD8+ T cells, suggesting potential anti-tumor activity.
Publisher
Communications Biology
Published On
Apr 22, 2024
Authors
My H. Hoang, Zachary L. Skidmore, Hans Rindt, Shirley Chu, Bryan Fisk, Jennifer A. Foltz, Catrina Fronick, Robert Fulton, Mingyi Zhou, Nathan J. Bivens, Carol N. Reinero, Todd A. Fehniger, Malachi Griffith, Jeffrey N. Bryan, Obi L. Griffith
Tags
T-cell receptor sequencing
dogs
cancer
immune response
melanoma
T-cell lymphoma

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