Single-cell T-cell receptor repertoire profiling in dogs | ResearchBunny

Index

Abstract

This study demonstrates the applicability of single-cell T-cell receptor sequencing (scTCRseq) in dogs with cancer. Researchers profiled PBMCs or lymph node aspirates from healthy dogs, dogs with T-cell lymphoma, and dogs with melanoma using scRNA and dog-specific scTCRseq primers. Healthy dogs showed highly diverse repertoires, lymphomas exhibited clonal repertoires, and melanoma dogs displayed a limited number of abundant clonotypes. Analysis of dominant clonotypes in melanoma PBMCs revealed predominantly activated CD8+ T cells, suggesting potential anti-tumor activity.

Publisher

Communications Biology

Published On

Apr 22, 2024

Authors

My H. Hoang, Zachary L. Skidmore, Hans Rindt, Shirley Chu, Bryan Fisk, Jennifer A. Foltz, Catrina Fronick, Robert Fulton, Mingyi Zhou, Nathan J. Bivens, Carol N. Reinero, Todd A. Fehniger, Malachi Griffith, Jeffrey N. Bryan, Obi L. Griffith

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