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Single-Cell Individualized Electroporation via Real-Time Impedance Monitoring

Medicine and Health

Single-Cell Individualized Electroporation via Real-Time Impedance Monitoring

Zhang

Explore cutting-edge research by Zhang, et al. on a revolutionary single-cell individualized electroporation method using a microelectrode array chip that enhances gene transfection efficiency while maintaining cell viability. This approach features real-time impedance monitoring and impressive results across various cell lines, paving the way for personalized electroporation techniques.

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Playback language: English
Abstract
This paper proposes a single-cell individualized electroporation (EP) method using a microelectrode array chip for efficient and safe gene transfection. Real-time impedance monitoring optimizes EP parameters, balancing perforation efficiency and cell viability. The method is validated across multiple cell lines (HeLa, MCF-7, 293T) demonstrating high transfection efficiency of EGFP plasmid. Impedance indicators characterize perforation and viability, enabling personalized EP.
Publisher
Microsystems & Nanoengineering
Published On
Authors
Zhang
Tags
electroporation
gene transfection
microelectrode array
cell viability
EGFP plasmid
real-time monitoring
personalized medicine

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