Real-time 4D tracking of airborne virus-laden droplets and aerosols

There is currently no real-time airborne virus tracking method, hindering the understanding of rapid virus changes and associated health impacts. Nano-digital in-line holographic microscopy (Nano-DIHM) is a lensless technology that can directly obtain the interference patterns of objects by recording the scattered light information originating from the objects. Here, we provide evidence for real-time physicochemical tracking of virus-laden droplets and aerosols in the air using desktop label-free Nano-DIHM. The virus interference patterns, as single and ensemble particles, were imaged by the Nano-DIHM with 32.5 ms resolution. The next-generation Stingray and Octopus software was used to automate object detection, characterization and classification from the recorded holograms. The detection system was demonstrated to detect active MS2 bacteriophages, inactivated SARS-CoV-2 and RNA fragments, and an MS2 mixture with metallic and organic compounds. This work demonstrates the feasibility of using Nano-DIHM to provide rapid virus detection to improve transmission management in real time.