This study introduces a novel real-time airborne virus tracking method using Nano-Digital in-line Holographic Microscopy (Nano-DIHM). The method enables the physicochemical tracking of virus-laden droplets and aerosols with 32.5 ms resolution, automatically detecting and characterizing particles using Stingray and Octopus software. The system successfully detected MS2 bacteriophages, inactivated SARS-CoV-2, RNA fragments, and mixtures with metallic and organic compounds. This technology offers potential for rapid virus detection and improved real-time transmission management.

Devendra Pal, Marc Amyot, Chen Liang, Parisa A. Ariya