Pulse oximetry values from 33,080 participants in the Apple Heart & Movement Study

Wearable devices that include pulse oximetry (SpO₂) sensing afford the opportunity to capture oxygen saturation measurements from large cohorts under naturalistic conditions. Here we report a cross-sectional analysis of 72 million SpO₂ values collected from 33,080 individual participants in the Apple Heart and Movement Study, stratified by age, sex, body mass index (BMI), home altitude, and other demographic variables. Measurements aggregated by hour of day into 24-h SpO₂ profiles exhibit similar circadian patterns for all demographic groups, being approximately sinusoidal with nadir near midnight local time, zenith near noon local time, and mean 0.89 lower saturation during overnight hours. Single SpO₂ measurements averaged for each subject into mean nocturnal and daytime SpO₂ values, employ multivariate ordinary least squares regression to quantify population-level trends according to demographic factors. For the full cohort, regression coefficients obtained from models fit to daytime SpO₂ are in close quantitative agreement with the corresponding values from published reference models for awake arterial oxygen saturation measured under controlled laboratory conditions. Regression models stratified by sex reveal significantly different age- and BMI-dependent SpO₂ trends for females compared with males, although constant terms and regression coefficients for altitude do not differ between sexes. Incorporating categorical variables encoding self-reported race/ethnicity into the full-cohort regression models identifies small but statistically significant differences in daytime SpO₂ (largest coefficient corresponding to 0.13% lower SpO₂ for Hispanic study participants compared to White participants), but no significant differences between groups for nocturnal SpO₂. Additional stratified analysis comparing regression models fit independently to subjects in each race/ethnicity group is suggestive of small differences in age- and sex-dependent trends, but indicates no significant difference in constant terms between any race/ethnicity groups for either daytime or nocturnal SpO₂. The large diverse study population and study employing extensive background SpO₂ measurements in well 24-h circadian cycles enables the establishment of healthy population norms.

Ian Shapiro, Jeff Stein, Calum MacRae, Michael O'Reilly