Plant growth promotion effect of plasma activated water on *Lactuca sativa* L. cultivated in two different volumes of substrate

Plasma activated water (PAW) can represent an alternative to chemical fertilizers in agriculture. The effects of PAW treatment applied in two concentrations (1.5 or 3.0 mg L⁻¹ NO₃) on some morphological, physiological, biochemical parameters and yield of Lactuca sativa L. grown in two different pot volumes (400 or 3200 cm³) were investigated in this study. The results showed that both PAW concentrations did not influence the germination, once the process was initiated. Positive effects of the treatments were registered on the length of radicle and hypocotyls of lettuce at a concentration of 1.5 mg L⁻¹ NO₃ (PAW I), the chlorophyll content was significantly increased at a concentration of 3.0 mg L⁻¹ NO₃⁻ (PAW II) and bigger pot volume, also the foliar weight and area. No significant differences between the treated and untreated plants were recorded for the root weight, leaf length and width. The dry weight was significantly higher for the lettuce treated with PAW I and II grown in big volume pots at 57 days after transplanting (DAT) and small volume pots at 64 DAT. The nitrites content of the lettuce grown in big pots was lower than of the lettuce grown in small pots, regardless of the PAW treatment. Contrary, the nitrates content was higher in the lettuce grown in big pots (up to 36.4 mg KNO₃/g DW), compared to small pots (under 0.3 mg KNO₃/g DW).

Vasile Stoleru, Radu Burlica, Gabriela Mihalache, Delicia Dirlau, Silvica Padureanu, Gabriel-Ciprian Teliban, Dragos Astanei, Alexandru Cojocaru, Oana Beniuga, Antoanela Patras