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Novel methods for global water safety monitoring: comparative analysis of low-cost, field-ready *E. coli* assays

Environmental Studies and Forestry

Novel methods for global water safety monitoring: comparative analysis of low-cost, field-ready *E. coli* assays

J. Brown, A. Bir, et al.

This study explores innovative and cost-effective methods for detecting *E. coli* in drinking water, addressing the critical need for reliable water safety testing, particularly in low-income regions. Conducted by Joe Brown, Arjun Bir, and Robert E. S. Bain, the research demonstrates the effectiveness of modified membrane filtration and presence-absence tests compared to standard practices, providing a viable solution for large-scale monitoring of water safety in Bangalore, India.

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Playback language: English
Abstract
Current microbiological water safety testing methods are often infeasible due to laboratory, cost, and other constraints, especially in low-income countries. This study evaluated two promising low-cost *E. coli* detection methods: a modified membrane filtration test using CompactDry™ plates and a presence-absence test using Aquatest (AT) medium. The results were compared to the standard membrane filtration with MI agar. Testing 315 drinking water samples in Bangalore, India, revealed high sensitivity and specificity for both candidate tests compared to the standard method, making them suitable for large-scale *E. coli* monitoring to assess water safety.
Publisher
npj Clean Water
Published On
Mar 30, 2020
Authors
Joe Brown, Arjun Bir, Robert E. S. Bain
Tags
E. coli detection
water safety
microbiological testing
cost-effective methods
drinking water
Bangalore
low-income countries
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