Flow virometry (FVM) offers near real-time viral water quality information, crucial for advanced water treatment and reuse. This study optimizes FVM protocols for T4 bacteriophage detection using a fractional factorial design, improving upon existing methods. It also explores density-based clustering (OPTICS algorithm) for automated data analysis, comparing it to manual gating. The optimized protocol and automated analysis pipeline, demonstrated on wastewater samples, enhance FVM's reliability and efficiency for microbial water quality monitoring.
Publisher
npj Clean Water
Published On
Apr 04, 2023
Authors
Hannah R. Safford, Melis M. Johnson, Heather N. Bischel
Tags
flow virometry
water quality
T4 bacteriophage
automated analysis
microbial monitoring
density-based clustering
wastewater
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