This study assessed the therapeutic potential of four rumen microbiome-derived antimicrobial peptides (AMPs) against *Pseudomonas aeruginosa*. All AMPs exhibited antimicrobial activity, with MICs ranging from 4–512 µg/mL. Time-kill kinetics showed complete kill within 10 min to 4 h. Biofilm formation was significantly inhibited, and no resistance development was observed. AMP cytotoxicity was minimal. The AMPs permeabilized the *P. aeruginosa* membrane, and transcriptome/metabolome analysis revealed increased catalytic activity at the cell membrane and β-oxidation of fatty acids. Lynronne 1 and 2 were efficacious in vivo, showing 100% survival in a *Galleria mellonella* infection model.
Publisher
npj Biofilms and Microbiomes
Published On
Aug 29, 2022
Authors
Adam J. Mulkern, Linda B. Oyama, Alan R. Cookson, Christopher J. Creevey, Toby J. Wilkinson, Hamza Olleik, Marc Maresca, Giarla C. da Silva, Patricia P. Fontes, Denise M. S. Bazzolli, Hilario C. Mantovani, Bamu F. Damaris, Luis A. J. Mur, Sharon A. Huws
Tags
antimicrobial peptides
Pseudomonas aeruginosa
rumen microbiome
in vivo
biological activity
membrane permeabilization
biofilm inhibition
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