Food Science and Technology

Microbiome-based environmental monitoring of a dairy processing facility highlights the challenges associated with low microbial-load samples

A. J. Mchugh, M. Yap, et al.

Explore groundbreaking findings from McHugh, Yap, Crispie, Feehily, Hill, and Cotter as they revolutionize food chain microbiome analysis with Oxford Nanopore Technologies MinION sequencing. This study demonstrates not only a competitive performance against Illumina sequencing but also highlights exciting prospects for rapid analysis in environmental monitoring.

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Abstract

This study assessed the use of Oxford Nanopore Technologies (ONT) MinION sequencing for rapid analysis of food chain microbiomes, comparing its performance with Illumina sequencing for environmental monitoring of a dairy processing facility. MinION sequencing accurately classified species to a level comparable to Illumina, demonstrating its potential for rapid analysis. However, the high DNA concentrations required for MinION library preparation posed a limitation, especially in low microbial-load environments, necessitating DNA amplification.

Publisher

npj Science of Food

Published On

Feb 15, 2021

Authors

Aoife J. McHugh, Min Yap, Fiona Crispie, Conor Feehily, Colin Hill, Paul D. Cotter

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