This paper reports the discovery of a fluorogenic small molecule substrate (QPD-OTf) for kinesin-1, a motor protein crucial for intracellular cargo transport. QPD-OTf produces a precipitating dye along kinesin-1's path on microtubules (MTs), enabling visualization of its native transport activity in live cells without external modifications. In vitro assays and docking studies support the substrate's binding in kinesin-1's nucleotide-binding site, and in cells, fluorescent trails reveal kinesin-1 transport from the Golgi.
Publisher
Nature Communications
Published On
Mar 05, 2021
Authors
Simona Angerani, Eric Lindberg, Nikolai Klena, Christopher K. E. Bleck, Charlotte Aumeier, Nicolas Winssinger
Tags
kinesin-1
fluorogenic substrate
microtubules
intracellular transport
visualization
live cells
cargo transport
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