Kinesin-1 activity recorded in living cells with a precipitating

Index

Abstract

This paper reports the discovery of a fluorogenic small molecule substrate (QPD-OTf) for kinesin-1, a motor protein crucial for intracellular cargo transport. QPD-OTf produces a precipitating dye along kinesin-1's path on microtubules (MTs), enabling visualization of its native transport activity in live cells without external modifications. In vitro assays and docking studies support the substrate's binding in kinesin-1's nucleotide-binding site, and in cells, fluorescent trails reveal kinesin-1 transport from the Golgi.

Publisher

Nature Communications

Published On

Mar 05, 2021

Authors

Simona Angerani, Eric Lindberg, Nikolai Klena, Christopher K. E. Bleck, Charlotte Aumeier, Nicolas Winssinger

Related Publications

Explore these studies to deepen your understanding of the subject.

Medicine and Health

Living with COPD and its psychological effects on participating in community-based physical activity in Brazil: a qualitative study. Findings from the Breathe Well group

S. M. Martins, R. Adams, et al.

Health and Fitness

Both sedentary time and physical activity are associated with cardiometabolic health in overweight adults in a 1-month accelerometer measurement

T. Sjöros, H. Vähä-ypyä, et al.

Medicine and Health

Identification of a new cannabidiol n-hexyl homolog in a medicinal cannabis variety with an antinociceptive activity in mice: cannabidihexol

P. Linciano, C. Citti, et al.

Medicine and Health

Effectiveness, Acceptability and Feasibility of Technology-Enabled Health Interventions for Adolescents Living with HIV in Low-and Middle-Income Countries: A Systematic Review

P. B. Tchounwou, C. A. Latkin, et al.

Listen, Learn & Level Up

Over 10,000 hours of research content in 25+ fields, available in 12+ languages.

No more digging through PDFs, just hit play and absorb the world's latest research in your language, on your time.

listen to research audio papers with researchbunny