Biology

Kinesin-1 activity recorded in living cells with a precipitating dye

S. Angerani, E. Lindberg, et al.

Discover a groundbreaking fluorogenic small molecule substrate, QPD-OTf, designed for kinesin-1, an essential motor protein for intracellular cargo transport. This innovative substrate enables visualization of kinesin-1's activity in live cells without any modifications, showcasing its path as it moves from the Golgi along microtubules. This exciting research was conducted by Simona Angerani, Eric Lindberg, Nikolai Klena, Christopher K. E. Bleck, Charlotte Aumeier, and Nicolas Winssinger.

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Abstract

This paper reports the discovery of a fluorogenic small molecule substrate (QPD-OTf) for kinesin-1, a motor protein crucial for intracellular cargo transport. QPD-OTf produces a precipitating dye along kinesin-1's path on microtubules (MTs), enabling visualization of its native transport activity in live cells without external modifications. In vitro assays and docking studies support the substrate's binding in kinesin-1's nucleotide-binding site, and in cells, fluorescent trails reveal kinesin-1 transport from the Golgi.

Publisher

Nature Communications

Published On

Mar 05, 2021

Authors

Simona Angerani, Eric Lindberg, Nikolai Klena, Christopher K. E. Bleck, Charlotte Aumeier, Nicolas Winssinger

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