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High-resolution landscape of an antibiotic binding site

Biology

High-resolution landscape of an antibiotic binding site

K. B. Yang, M. Cameranesi, et al.

This groundbreaking research by Kevin B. Yang and colleagues reveals a collection of 760 single-residue mutants in *Escherichia coli* RNA polymerase, significantly influencing rifampicin binding and converting bacteriostatic effects to lethal action. The study uncovers mutations that enhance RNAP speed and examines natural sequence diversity, paving the way for novel antibiotic insights.

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