Full spectrum fluorescence lifetime imaging with 0.5 nm spectral and 50 ps temporal resolution

This paper presents a high-speed optical scanning microscope capable of capturing time-resolved images across 512 spectral and 32 time channels in a single acquisition, achieving a potential frame rate of -0.2 frames per second (256 × 256 image pixels). The system overcomes data volume and low imaging speed challenges through integrated system control electronics and on-chip processing. It enables the creation of detailed data cubes for each pixel, facilitating the study of diverse time-resolved light-driven phenomena. The system's performance is demonstrated through imaging various samples, including human lung tissue, showcasing its potential for analyzing complex organic structures and revealing subtle spectral or lifetime changes.

Gareth O. S. Williams, Elvira Williams, Neil Finlayson, Ahmet T. Erdogan, Qiang Wang, Susan Fernandes, Ahsan R. Akram, Kev Dhaliwal, Robert K. Henderson, John M. Girkin, Mark Bradley