Electrochemical degradation and saponification of porcine adipose tissue

Body contouring achieved via subcutaneous adipose tissue reduction has notably advanced over the past century, from suction assisted lipectomy to techniques with reduced degrees of invasiveness including laser, radiofrequency, high frequency focused ultrasound, cryolipolysis, and drug-based injection approaches. These costly techniques have focused on damaging adipocyte cell membranes, hydrolyzing triglycerides (TGs), or inducing apoptosis. Here, we present a simple, low-cost technique, termed electrochemical lipolysis (ECLL). During ECLL, saline is injected into the subcutaneous adipose tissue, followed by insertion of needle electrodes and application of an electrical potential. Electrolysis of saline creates localized pH gradients that drive adipocyte death and saponification of TGs. Using pH mapping, various optical imaging techniques, and biochemical assays, we demonstrate the ability of ECLL to induce acid and base injury, cell death, and the saponification of triglycerides in ex vivo porcine adipose tissue. We define ECLL's potential role as a minimally-invasive, ultra-low-cost technology for reducing and contouring adipose tissue, and present ECLL as a potential new application of an emerging electrochemical redox based treatment modality.

Tiffany T. Pham, Anna M. Stokolosa, Pamela A. Borden, Kyle D. Hansen, Ellen M. Hong, Tatiana B. Krasieva, Ryan H. Sivoraphonh, Wesley J. Moy, Andrew E. Heidari, Lauren H. Lee, Eun-Hee Kim, Chung-Ho Sun, Wangcun Jia, Ji-Hun Mo, Sehwan Kim, Michael G. Hill, Brian J. F. Wong