Food Science and Technology

Dominance of phage particles carrying antibiotic resistance genes in the viromes of retail food sources

P. Blanco-picazo, S. Morales-cortes, et al.

Explore the exciting findings of a study that uncovers the significant roles of phages, outer membrane vesicles, and contaminating DNA in antibiotic resistance genes within food viromes. Conducted by a team of researchers including Pedro Blanco-Picazo and Sara Morales-Cortes, this research reveals the intricate dynamics of viromes in our food systems.

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Abstract

This study investigated the contribution of phages, outer membrane vesicles (OMVs), and contaminating DNA to the presence of antibiotic resistance genes (ARGs) in food viromes. Viral fractions from chicken, fish, and mussels were analyzed. After purification using CsCl density gradient centrifugation and DNase treatment, the presence of ARGs within phage particles was confirmed. Proteomic analysis revealed a predominance of phage proteins (71.8% in fish, 52.9% in mussels, 78.7% in chicken sample 1, and 64.1% in chicken sample 2), while membrane proteins (3.8–21.4%) were less abundant, indicating minimal OMV contamination. Electron microscopy confirmed the presence of Myoviridae and Siphoviridae phages. The results support the reliability of methods used to detect ARG-carrying phage particles and highlight their significance in food viromes.

Publisher

The ISME Journal

Published On

Oct 26, 2022

Authors

Pedro Blanco-Picazo, Sara Morales-Cortes, María Dolores Ramos-Barbero, Cristina García-Aljaro, Lorena Rodríguez-Rubio, Maite Muniesa

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