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Cytosine base editor 4 but not adenine base editor generates off-target mutations in mouse embryos

Biology

Cytosine base editor 4 but not adenine base editor generates off-target mutations in mouse embryos

H. K. Lee, H. E. Smith, et al.

This groundbreaking study by Hye Kyung Lee and colleagues delves into the fidelity of cytosine base editor 4 (BE4) versus adenine base editor (ABE) in mouse embryos, revealing critical insights about genetic editing efficiency and precision.

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Playback language: English
Abstract
This study investigates the fidelity of cytosine base editor 4 (BE4) and adenine base editor (ABE) in mouse embryos using whole-genome sequencing. The results show that BE4-edited mice have more single-nucleotide variants and deletions than ABE-edited mice and controls, indicating a need for BE4 optimization. While ABE demonstrates high fidelity, rare aberrant C-to-T conversions at specific target sites require further investigation.
Publisher
Communications Biology
Published On
Jan 09, 2020
Authors
Hye Kyung Lee, Harold E. Smith, Chengyu Liu, Michaela Willi, Lothar Hennighausen
Tags
cytosine base editor
adenine base editor
mouse embryos
whole-genome sequencing
genetic editing
fidelity
single-nucleotide variants

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