Biology

CRISPR-Cas13d effectively targets SARS-CoV-2 variants, including Delta and Omicron, and inhibits viral infection

Z. Liu, X. Gao, et al.

This groundbreaking study reveals a novel CRISPR-Cas13d-based strategy with over 99% efficiency in targeting SARS-CoV-2 variants, including Delta and Omicron. Conducted by a team of experts including Zongzhi Liu, Xiang Gao, and others, this research highlights the rapid antiviral potential of this approach, paving the way for future defenses against viral threats.

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Abstract

The emergence of SARS-CoV-2 variants necessitates innovative antiviral strategies. This study demonstrates the effectiveness of a CRISPR-Cas13d-based approach in degrading viral RNA. crRNAs targeting conserved regions of NSP13, NSP14, and nucleocapsid transcripts achieved >99% silencing efficiency in human cells infected with various SARS-CoV-2 variants, including Delta and Omicron. Bioinformatics analysis showed the crRNAs could target almost 100% of the SARS-CoV family. The high specificity, efficiency, and rapid deployment potential of this system highlight its promise for antiviral drug development and protection against future variants.

Publisher

Molecular Therapy - Oncolytics

Published On

Jan 01, 2023

Authors

Zongzhi Liu, Xiang Gao, Chuanwen Kan, Lingyu Li, Yuan Zhang, Yibo Gao, Shengyuan Zhang, Liangji Zhou, Hui Zhao, Mingkun Li, Yingli Sun, Zheng Zhang

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