logo
ResearchBunny Logo
COV2-ID, a MIQE-compliant sub-20-min 5-plex RT-PCR assay targeting SARS-CoV-2 for the diagnosis of COVID-19

Medicine and Health

COV2-ID, a MIQE-compliant sub-20-min 5-plex RT-PCR assay targeting SARS-CoV-2 for the diagnosis of COVID-19

S. Bustin, A. Coward, et al.

Discover the groundbreaking CoV2-ID assay developed by Stephen Bustin and colleagues! This rapid, five-plex RT-qPCR test offers 100% sensitivity and specificity in detecting SARS-CoV-2 with results in just 15 minutes. A must-listen for anyone interested in cutting-edge COVID-19 research!

00:00
00:00
Playback language: English
Abstract
This research paper details the development and validation of CoV2-ID, a rapid and highly sensitive five-plex RT-qPCR assay for the detection of SARS-CoV-2. The assay targets three viral genes (Nsp10, Nsp12, and N), includes a human internal control, and an artificial RNA control for quality assessment. CoV2-ID demonstrates 100% sensitivity and specificity, with a limit of detection of a single viral RNA copy and a run time of approximately 15 minutes. The assay also incorporates a mutation detection component for the D614G mutation.
Publisher
Scientific Reports
Published On
Dec 17, 2020
Authors
Stephen Bustin, Amy Coward, Garry Sadler, Louise Teare, Tania Nolan
Tags
SARS-CoV-2
RT-qPCR
diagnostic assay
mutation detection
viral RNA

Related Publications

Explore these studies to deepen your understanding of the subject.

Risk factors for and pregnancy outcomes after SARS-CoV-2 in pregnancy according to disease severity: A nationwide cohort study with validation of the SARS-CoV-2 diagnosis of Nordic Federation of Societies of Obstetrics and Gynecology (NFOG)
Medicine and Health

Risk factors for and pregnancy outcomes after SARS-CoV-2 in pregnancy according to disease severity: A nationwide cohort study with validation of the SARS-CoV-2 diagnosis of Nordic Federation of Societies of Obstetrics and Gynecology (NFOG)

A. J. M. Aabakke, T. G. Petersen, et al.

The COVID-AGICT study: COVID-19 and advanced gastro-intestinal cancer surgical treatment. A multicentric Italian study on the SARS-CoV-2 pandemic impact on gastro-intestinal cancers surgical treatment during the 2020. Analysis of perioperative and short-term oncological outcomes
Medicine and Health

The COVID-AGICT study: COVID-19 and advanced gastro-intestinal cancer surgical treatment. A multicentric Italian study on the SARS-CoV-2 pandemic impact on gastro-intestinal cancers surgical treatment during the 2020. Analysis of perioperative and short-term oncological outcomes

G. Giuliani, F. Guerra, et al.

Collaboration in the time of COVID: a scientometric analysis of multidisciplinary SARS-CoV-2 research
Interdisciplinary Studies

Collaboration in the time of COVID: a scientometric analysis of multidisciplinary SARS-CoV-2 research

E. Cunningham, B. Smyth, et al.

A nanoluciferase SARS-CoV-2 for rapid neutralization testing and screening of anti-infective drugs for COVID-19
Medicine and Health

A nanoluciferase SARS-CoV-2 for rapid neutralization testing and screening of anti-infective drugs for COVID-19

X. Xie, A. E. Muruato, et al.

Listen, Learn & Level Up
Over 10,000 hours of research content in 25+ fields, available in 12+ languages.
No more digging through PDFs, just hit play and absorb the world's latest research in your language, on your time.
listen to research audio papers with researchbunny