Biology

Connectome-seq: High-throughput Mapping of Neuronal Connectivity at Single-Synapse Resolution via Barcode Sequencing

D. Chen, A. Isakova, et al.

Connectome-seq is a high-throughput AAV-based method that maps neuronal connectivity at single-synapse resolution by combining engineered synaptic proteins, RNA barcoding, and parallel single-nucleus and single-synaptosome sequencing to capture both synaptic links and molecular identities. Research conducted by Danping Chen, Alina Isakova, Zhou Joe Wan, Mark J. Wagner, Yunming Wu, and Boxuan Simen Zhao.