Adding adipose cells to cell-cultured meat can improve its aroma and texture. This study presents an immortalized porcine preadipocyte cell strain (ISP-4) that can be cultured for over 40 passages without losing its adipogenic potential. ISP-4 efficiently differentiates within 3D bioscaffolds using fewer chemicals and less serum, and can be upscaled using microcarriers. It also co-differentiates with muscle precursor cells, creating a meat-like structure. ISP-4 is a promising model for studying and producing cell-cultured fat.
