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A rapid colorimetric LAMP assay for detection of *Rhizoctonia solani* AG-1 IA causing sheath blight of rice

Agriculture

A rapid colorimetric LAMP assay for detection of *Rhizoctonia solani* AG-1 IA causing sheath blight of rice

P. Choudhary, P. Rai, et al.

Discover the rapid detection of *Rhizoctonia solani*, a major rice pathogen, through a groundbreaking colorimetric LAMP assay. This innovative method allows for confirmation of the pathogen in just 45 minutes, demonstrating high sensitivity and specificity. This research offers a crucial point-of-care diagnostic tool for timely intervention, conducted by Prassan Choudhary, Pallavi Rai, Jagriti Yadav, Shaloo Verma, Hillol Chakdar, Sanjay Kumar Goswami, Alok Kumar Srivastava, Prem Lal Kashyap, and Anil Kumar Saxena.

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Playback language: English
Abstract
*Rhizoctonia solani* is a devastating pathogen causing sheath blight in rice. This study developed a colorimetric LAMP assay for its rapid detection. Four primers targeting the polygalacturonase (PG) gene showed specific amplification, with q-PCR detecting up to 10 pg of DNA. A colorimetric LAMP assay, coupled with a rapid template preparation method, visually confirmed the pathogen within 45 minutes. The LAMP assay's sensitivity was 1.65 fg/µl, effectively detecting *R. solani* AG-1 IA from diseased tissues and soil. High specificity was demonstrated by the lack of amplification with other AG groups and related fungi/bacteria. This assay offers a point-of-care diagnostic tool for early monitoring and preventive measures.
Publisher
Scientific Reports
Published On
Dec 16, 2020
Authors
Prassan Choudhary, Pallavi Rai, Jagriti Yadav, Shaloo Verma, Hillol Chakdar, Sanjay Kumar Goswami, Alok Kumar Srivastava, Prem Lal Kashyap, Anil Kumar Saxena
Tags
Rhizoctonia solani
sheath blight
colorimetric LAMP assay
rapid detection
polygalacturonase gene
pathogen diagnostics
sensitivity and specificity

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