This paper introduces a microphysiological system designed to record the real-time barrier permeability of mouse colon tissue in a physiological environment over extended durations. The system utilizes a microfluidic chamber, media preserving the microbiome and creating oxygen gradients, and integrated sensor electrodes for acquiring transepithelial electrical resistance (TEER). The system maintains tissue viability for up to 72 hours and can distinguish permeability levels under various treatments, demonstrating its potential for studying leaky gut and other barrier-related diseases.

Ryan Way, Hayley Templeton, Daniel Ball, Ming-Hao Cheng, Stuart A. Tobet, Thomas Chen