Biology

A generalizable nanopore sensor for highly specific protein detection at single-molecule precision

M. Ahmad, J. Ha, et al.

Discover groundbreaking nanopore sensors developed by Mohammad Ahmad and colleagues for ultra-specific protein detection at a single molecule level. These innovative sensors, featuring a unique programmable binder, enable label-free, real-time analysis, opening new doors in biomedical diagnostics for biomarker detection in biofluids.

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Abstract

This paper presents a novel class of nanopore sensors for highly specific protein detection at single-molecule precision. The sensors utilize a programmable antibody-mimetic binder fused to a monomeric protein nanopore, enabling detection of proteins with varying size, charge, and structural complexity. The approach overcomes steric hindrance challenges and allows for label-free, real-time detection with high sensitivity and specificity. The developed sensors show potential for application in biomedical diagnostics for biomarker detection in biofluids.

Publisher

Nature Communications

Published On

Mar 20, 2023

Authors

Mohammad Ahmad, Jeung-Hoi Ha, Lauren A. Mayse, Maria F. Presti, Aaron J. Wolfe, Kelsey J. Moody, Stewart N. Loh, Liviu Movileanu

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