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Abstract
Single-molecule fluorescence detection typically requires expensive optical instruments. This paper introduces NanoAntennas with Cleared HotSpots (NACHOS), DNA origami nanostructures designed for bioassay incorporation, enhancing single-emitter fluorescence up to 461-fold. These allow detection using a smartphone camera and an inexpensive objective lens. A portable smartphone microscope was built and successfully used for a single-molecule DNA detection assay for antibiotic-resistant *Klebsiella pneumoniae*, demonstrating the potential for affordable point-of-care diagnostics.
Publisher
Nature Communications
Published On
Feb 11, 2021
Authors
Kateryna Trofymchuk, Viktorija Glembockyte, Lennart Grabenhorst, Florian Steiner, Carolin Vietz, Cindy Close, Martina Pfeiffer, Lars Richter, Max L. Schütte, Florian Selbach, Renukka Yaadav, Jonas Zähringer, Qingshan Wei, Aydogan Ozcan, Birka Lalkens, Guillermo P. Acuna, Philip Tinnefeld
Tags
single-molecule fluorescence
DNA origami
bioassay
smartphone microscope
point-of-care diagnostics
antibiotic resistance
Klebsiella pneumoniae

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