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A diverse proteome is present and enzymatically active in metabolite extracts

Biology

A diverse proteome is present and enzymatically active in metabolite extracts

R. (. J. House, M. T. Soper-hopper, et al.

Discover groundbreaking findings in metabolomics by Rachel (Rae) J. House, Molly T. Soper-Hopper, and colleagues, revealing a surprising proteome presence even in metabolite extracts. This study highlights the refined extraction workflow that tackles protein interferences, enhancing the accuracy of metabolic analyses.

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Playback language: English
Abstract
Metabolite extraction, a crucial initial step in metabolomics, is generally assumed to inactivate and remove proteins. This study reveals a diverse proteome (over 1000 proteins) within metabolite extracts across various extraction methods and sample types. Residual transaminase activity in extracts leads to metabolite interconversions, demonstrated by in-extract glutamate dipeptide formation and glutathione depletion. A refined extraction workflow incorporating 3 kDa filtration for protein removal is proposed as a superior method for polar metabolomics, mitigating protein-mediated observer effects in metabolomics experiments.
Publisher
Nature Communications
Published On
Jul 10, 2024
Authors
Rachel (Rae) J. House, Molly T. Soper-Hopper, Michael P. Vincent, Abigail E. Ellis, Colt D. Capan, Zachary B. Madaj, Emily Wolfrum, Christine N. Isaguirre, Carlos D. Castello, Amy B. Johnson, Martha L. Escobar Galvis, Kelsey S. Williams, Hyoungjoo Lee, Ryan D. Sheldon
Tags
metabolite extraction
proteome
metabolomics
transaminase activity
glutamine depletion
polar metabolomics

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