Developing new rice varieties with both high yield and good quality is crucial for food security. This study uses comparative transcriptomics and genomic analysis of the hybrid rice variety Chuanyou6203 (CY6203) and its parents to investigate the molecular mechanisms governing yield and quality. Analysis revealed numerous differentially expressed genes (DEGs) and variations between parents, suggesting dominance or epistasis might contribute to CY6203's heterosis. The study identifies key gene modules associated with yield (photosynthesis, nitrogen utilization) and quality traits, providing insights into the molecular basis of CY6203's superior characteristics.