Biology

Synthetic zwitterions as efficient non-permeable cryoprotectants

Y. Kato, T. Uto, et al.

Discover how synthetic zwitterions can revolutionize cryopreservation techniques for cells. This research, conducted by Yui Kato, Takuya Uto, Daisuke Tanaka, and their colleagues, reveals that combining zwitterions with DMSO enhances cell viability beyond traditional cryoprotectants, particularly for cells vulnerable to freezing damage.

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Abstract

This paper investigates the use of synthetic zwitterions as cryoprotectants for cells. The authors synthesized 18 zwitterion species and assessed their cryoprotective effects. Non-permeable zwitterions inhibit ice crystal formation, but some cells remained insufficiently dehydrated. Combining zwitterions with DMSO improved cryopreservation, yielding higher cell viability than commercial cryoprotectants, especially for freezing-vulnerable cells. Molecular dynamics simulations suggested that zwitterions protect cell membranes from DMSO-induced collapse.

Publisher

Communications Chemistry

Published On

Oct 28, 2021

Authors

Yui Kato, Takuya Uto, Daisuke Tanaka, Kojiro Ishibashi, Akiko Kobayashi, Masaharu Hazawa, Richard W. Wong, Kazuaki Ninomiya, Kenji Takahashi, Eishu Hirata, Kosuke Kuroda

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