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Abstract
Fucosylation is crucial for various protein functions. AlfC, an α-fucosidase from *Lactobacillus casei*, displays high specificity for α(1,6)-fucose linkages. This study uses crystal structures, mutational and kinetic analyses, and molecular dynamics to elucidate AlfC's mechanism. Results suggest an aromatic subsite for GlcNAc accommodation, activity control via active-site loop conformations, and a framework for creating AlfC transfucosidase mutants.
Publisher
NATURE COMMUNICATIONS
Published On
Dec 04, 2020
Authors
Erik H. Klontz, Chao Li, Kyle Kihn, James K. Fields, Dorothy Beckett, Greg A. Snyder, Patrick L. Wintrode, Daniel Deredge, Lai-Xi Wang, Eric J. Sundberg
Tags
fucosylation
α-fucosidase
Lactobacillus casei
crystal structures
molecular dynamics

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