Agriculture

Split selectable marker systems utilizing inteins facilitate gene stacking in plants

G. Yuan, H. Lu, et al.

Discover a groundbreaking method for stacking multiple genes in plants, utilizing split selectable marker systems with inteins for *Agrobacterium*-mediated co-transformation. This innovative approach not only reconstitutes a visible marker in tobacco but also facilitates the stacking of reporters in *Arabidopsis* and poplar, revolutionizing plant co-transformation. This significant research was conducted by Guoliang Yuan, Haiwei Lu, Kuntal De, Md Mahmudul Hassan, Yang Liu, Md. Torikul Islam, Wellington Muchero, Gerald A. Tuskan, and Xiaohan Yang.

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Abstract

The ability to stack multiple genes in plants is crucial for developing crops with desirable traits but faces challenges due to limited selectable marker options. This study establishes split selectable marker systems using inteins for *Agrobacterium*-mediated co-transformation. The effectiveness of the system is demonstrated by reconstituting a visible marker, RUBY, in tobacco and stacking two reporters (eYGFPuv and RUBY) in *Arabidopsis* and poplar using split Kanamycin or Hygromycin resistance markers. This method enables robust plant co-transformation, providing a valuable tool for efficient multi-gene insertion.

Publisher

Communications Biology

Published On

May 26, 2023

Authors

Guoliang Yuan, Haiwei Lu, Kuntal De, Md Mahmudul Hassan, Yang Liu, Md. Torikul Islam, Wellington Muchero, Gerald A. Tuskan, Xiaohan Yang

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