Emmer wheat (*Triticum turgidum* subsp. *dicoccum* and *dicoccoides*), durum wheat (*T. turgidum* subsp. *durum*), *T. timopheevii*, and D genome-containing *Aegilops* species are valuable sources of genetic variation for improving bread wheat (*T. aestivum*). This study created 192 cross combinations between diverse wheat and *Aegilops* genotypes, resulting in 56 synthetic hexaploid and octaploid F2 lines with AABBDD, AABBDDDD, AAGGDD, D'D¹XXAABB, D'D¹C¹CAABB, and D'D¹N¹N¹AABB genomes. Cytogenetic analysis confirmed the produced lines as stable amphiploids, demonstrating the potential of emmer wheat and *Aegilops* species for broadening bread wheat genetic variation.