Agriculture

Precise in-field molecular diagnostics of crop diseases by smartphone-based mutation-resolved pathogenic RNA analysis

T. Zhang, Q. Zeng, et al.

Discover a groundbreaking molecular diagnostic tool developed by Ting Zhang and colleagues that revolutionizes crop disease detection. This innovative method uses a colorimetric paper and smartphone technology for rapid, low-cost multiplexed pathogen identification, all within 10 minutes! Gain insights into fungal RNA detection and resistance mutations for precision crop management.

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Playback language: English

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Abstract

This paper presents a novel in-field molecular diagnostic tool for crop diseases using a colorimetric paper and a smartphone. The tool enables multiplexed, low-cost, and rapid detection of crop pathogens via a nucleic acid amplification-free method combining toehold-mediated strand displacement with metal ion-mediated urease catalysis. The assay can distinguish viable and dead pathogens and identify fungicide-resistant isolates by detecting fungal RNA and mutations, providing crucial information for precision crop disease management. The entire sample-to-result process, including microneedle-based nucleic acid extraction and smartphone app analysis, takes approximately 10 minutes.

Publisher

Nature Communications

Published On

Jul 19, 2023

Authors

Ting Zhang, Qingdong Zeng, Fan Ji, Honghong Wu, Rodrigo Ledesma-Amaro, Qingshan Wei, Hao Yang, Xuhan Xia, Yao Ren, Keqing Mu, Qiang He, Zhensheng Kang, Ruijie Deng

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