Three-dimensional (3D) volume imaging of biological structures is increasingly important, but tissue opacity hinders deep tissue imaging. Existing optical clearing methods, while offering advantages, suffer from drawbacks like low transparency, slow processing, tissue deformation, or fluorescence quenching. This paper introduces OptiMuS, a novel single-step optical clearing solution that combines optimized concentrations of urea, iohexol, and sorbitol. OptiMuS achieves rapid and high transparency of various tissues and organs, while preserving tissue size and fluorescent signals. It's compatible with lipophilic dyes, enabling 3D visualization of vascular structures. Furthermore, OptiMuS, combined with 3D morphological analysis software, facilitates quantitative analysis of structures like glomeruli in kidneys.
