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Optimization of adeno-associated viral vector-mediated transduction of the corticospinal tract: comparison of four promoters

Medicine and Health

Optimization of adeno-associated viral vector-mediated transduction of the corticospinal tract: comparison of four promoters

B. Nieuwenhuis, B. Haenzi, et al.

This exciting research by Bart Nieuwenhuis and colleagues explores the effectiveness of various promoters in AAV1 for targeted gene delivery to the brain and spinal cord. Discover how mPGK and hSYN enhance expression in specific neuronal populations, paving the way for advancements in spinal cord injury treatment.

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Playback language: English
Abstract
Adeno-associated viral vectors (AAVs) are widely used for gene transfer to the nervous system. This study compares four promoters (sCAG, hCMV, mPGK, hSYN) packaged in AAV1 for corticospinal tract transduction in rats and mice. hCMV and sCAG resulted in broad expression, while mPGK and hSYN showed stronger, more targeted expression. mPGK drove expression in neurons and oligodendrocytes, whereas hSYN yielded neuron-specific expression, including in layer V corticospinal neurons. This optimized transduction improves gene delivery to the brain and spinal cord, benefiting spinal cord injury research.
Publisher
Gene Therapy
Published On
Jun 23, 2020
Authors
Bart Nieuwenhuis, Barbara Haenzi, Sam Hilton, Alejandro Carnicer-Lombarte, Barbara Hobo, Joost Verhaagen, James W. Fawcett
Tags
Adenovirus
gene transfer
AAV1
corticospinal tract
promoters
neurons
spinal cord injury

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