Biology

Membrane translocation process revealed by in situ structures of type II secretion system secretins

Z. Yu, Y. Wu, et al.

This research conducted by Zhili Yu, Yaoming Wu, Muyuan Chen, Tong Huo, Wei Zheng, Steven J. Ludtke, Xiaodong Shi, and Zhao Wang delves into the membrane translocation process of secretins GspDα and GspDβ in *Escherichia coli*. Through innovative electron cryotomography techniques, the distinct interaction patterns and models for these secretins provide new insights into T2SS biogenesis.

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Abstract

This research paper investigates the membrane translocation process of two types of secretins, GspDα and GspDβ, in *Escherichia coli*. Using electron cryotomography subtomogram averaging, the authors determine in situ structures of key intermediate states during translocation, revealing distinct membrane interaction patterns and peptidoglycan layer transitions for each secretin type. Two distinct models for GspDα and GspDβ membrane translocation are proposed, providing a comprehensive understanding of T2SS secretin biogenesis.

Publisher

Nature Communications

Published On

Jul 07, 2023

Authors

Zhili Yu, Yaoming Wu, Muyuan Chen, Tong Huo, Wei Zheng, Steven J. Ludtke, Xiaodong Shi, Zhao Wang

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