Vine tea (*Ampelopsis grossedentata*) extract (VTE) and its active component, dihydromyricetin (DMY), exhibit antibacterial properties against *Staphylococcus aureus*. This study investigated the mechanism of their action using various assays. SEM and TEM showed morphological changes in *S. aureus* after treatment. Membrane integrity disruption was indicated by AKPase and β-galactosidase leakage. VTE and DMY inhibited total protein expression and reduced energy metabolism enzyme activities. DNA binding assays revealed interactions through groove and intercalation. In food systems (cabbage and barley), VTE and DMY effectively inhibited bacterial growth, suggesting their potential as food preservatives.

Haiyun Liang, Keke He, Ting Li, Shumei Cui, Meng Tang, Shaoyi Kang, Wei Ma, Liya Song