Medicine and Health

GlomSpheres as a 3D co-culture spheroid model of the kidney glomerulus for rapid drug-screening

J. Tuffin, M. Chesor, et al.

Discover GlomSpheres, an innovative 3D co-culture spheroid model developed by Jack Tuffin and colleagues, designed to revolutionize drug screening for kidney glomerulus research. This model showcases organized cellular structures and responds dynamically to treatment, offering exciting new avenues for understanding renal health.

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Abstract

Current 2D and 3D models of the kidney glomerulus are imperfect for drug screening. This paper introduces GlomSpheres, a rapidly generated and reproducible 3D co-culture spheroid model. Using magnetic spheroid formation, human podocytes and glomerular endothelial cells (GEnCs) form organized collagen IV and laminin. Key podocyte and GEnC markers are upregulated, and electron microscopy shows foot process interdigitation and vessel formation. Treatment with profibrotic agents induces ECM dysregulation and podocyte loss, attenuated by Nintedanib. Incubation with patient plasma shows podocyte loss and ECM dysregulation, also reduced by Nintedanib. A rapid imaging approach demonstrates GlomSpheres' utility in high-throughput pharmaceutical screening.

Publisher

Communications Biology

Published On

Dec 02, 2021

Authors

Jack Tuffin, Musleeha Chesor, Valeryia Kuzmuk, Tim Johnson, Simon C. Satchell, Gavin I. Welsh, Moin A. Saleem

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