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Functionalizing tandem mass tags for streamlining click-based quantitative chemoproteomics

Biology

Functionalizing tandem mass tags for streamlining click-based quantitative chemoproteomics

N. R. Burton and K. M. Backus

Explore the innovative silane-based cleavable linkers for isotopically-labeled proteomics-tandem mass tag (sCIP-TMT) platform, developed by Nikolas R. Burton and Keriann M. Backus. This cutting-edge technology enables efficient sample preparation, achieving high throughput and coverage while accurately quantifying results in chemoproteomic applications. Unlock the potential of identifying covalent interactions with a remarkable capacity for detecting liganded cysteines.

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Playback language: English
Abstract
This paper introduces the silane-based cleavable linkers for isotopically-labeled proteomics-tandem mass tag (sCIP-TMT) proteomic platform. sCIP-TMT enables high-throughput and high-coverage sample preparation by employing early sample pooling, pairing a custom click-compatible sCIP capture reagent with commercially available TMT reagents. Benchmarking of a 10-plex sCIP-TMT set demonstrates reduced sample preparation time, high coverage, and accurate quantification. The platform's utility is shown through a chemoproteomic screen identifying 789 liganded cysteines, highlighting its potential for various covalent chemoproteomic applications.
Publisher
Communications Chemistry
Published On
Apr 10, 2024
Authors
Nikolas R. Burton, Keriann M. Backus
Tags
silanes
proteomics
tandem mass tag
sample preparation
chemoproteomics
quantification
cysteines

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