This paper describes force generation by a propagating wave of supramolecular nanofibers. A reaction network was designed to control the formation and degradation of nanofibers using two orthogonal stimuli. Real-time fluorescent imaging visualized the propagating wave. Numerical simulation showed that the concentration gradient of the degradation stimulus and the lower diffusion coefficient of the nanofiber were crucial for wave emergence. The generated force (0.005 pN) moved nanobeads.