Biology

Fast viral dynamics revealed by microsecond time-resolved cryo-EM

O. F. Harder, S. V. Barrass, et al.

This groundbreaking research by Oliver F. Harder, Sarah V. Barrass, Marcel Drabbels, and Ulrich J. Lorenz reveals how microsecond time-resolved cryo-electron microscopy captures the rapid pH-induced contraction of the cowpea chlorotic mottle virus capsid, showcasing remarkable details of protein dynamics that were previously beyond reach.

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Abstract

This research demonstrates the use of microsecond time-resolved cryo-electron microscopy (cryo-EM) to observe fast protein dynamics, specifically focusing on the pH-induced contraction of the cowpea chlorotic mottle virus (CCMV) capsid. The study reveals the microsecond timescale of this contraction, highlighting the concerted yet multi-timescale motions of capsid proteins. This technique offers unprecedented detail in observing protein dynamics previously inaccessible, promising significant advancements in understanding protein function.

Publisher

Nature Communications

Published On

Sep 13, 2023

Authors

Oliver F. Harder, Sarah V. Barrass, Marcel Drabbels, Ulrich J. Lorenz

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