Voltage imaging, while promising for biomedical research, is limited by the need for high-speed, volume-imaging capabilities. Current remote focusing techniques are either too slow or light-inefficient. This paper introduces flipped image remote focusing (FLIRF), a method doubling light efficiency compared to conventional techniques, enabling volumetric voltage imaging at 500 volumes/s. The method's potential is demonstrated by recording activity from over 100 neurons in a zebrafish spinal cord using light sheet imaging.

Urs L. Böhm, Benjamin Judkewitz