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DNA storage in thermoresponsive microcapsules for repeated random multiplexed data access

Biology

DNA storage in thermoresponsive microcapsules for repeated random multiplexed data access

B. W. A. Bögels, B. H. Nguyen, et al.

Discover a groundbreaking method for multiplexed, random access to DNA files, utilizing thermoconfined PCR techniques. This innovative approach, developed by a team of expert researchers, significantly reduces amplification bias while enhancing storage scalability and efficiency.

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Playback language: English
Abstract
This paper presents a novel thermoconfined polymerase chain reaction (PCR) method for multiplexed, repeated random access to compartmentalized DNA files. Biotin-functionalized oligonucleotides are localized within thermoresponsive, semipermeable microcapsules (proteinosomes). At low temperatures, the capsules are permeable, allowing enzymes and primers access; at high temperatures, membrane collapse prevents crosstalk during amplification. The method demonstrates superior performance compared to non-compartmentalized DNA storage, reducing amplification bias and enabling scalable, sequence-agnostic repeated access.
Publisher
Nature Nanotechnology
Published On
Aug 01, 2023
Authors
Bas W. A. Bögels, Bichlien H. Nguyen, David Ward, Levena Gascoigne, David P. Schrijver, Anna-Maria Makri Pistikou, Alex Joesaar, Shuo Yang, Ilja K. Voets, Willem J. M. Mulder, Andrew Phillips, Stephen Mann, Georg Seelig, Karin Strauss, Yuan-Jyue Chen, Tom F. A. de Greef
Tags
thermoconfined PCR
DNA storage
compartmentalized amplification
biotin-functionalized oligonucleotides
thermoresponsive microcapsules
enzyme access
repeated random access

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