This paper introduces DaXi, a single-objective light-sheet microscope designed to combine the ease of use of standard single-objective microscopes with the advantages of light-sheet microscopy. DaXi achieves high-resolution imaging over a large field of view (achieving 450 nm lateral and 2 µm axial resolution over a 3,000 × 800 × 300 µm volume), fast volumetric imaging of large samples without tiling, full image coverage through multi-view imaging, and high-throughput multi-well imaging. Its capabilities are demonstrated through imaging various biological systems, including Drosophila egg chamber development, zebrafish whole-brain activity, and zebrafish embryonic development.

Bin Yang, Merlin Lange, Alfred Millett-Sikking, Xiang Zhao, Jordão Bragantini, Shruthi Vijay Kumar, Mason Kamb, Rafael Gómez-Sjöberg, Ahmet Can Solak, Wanpeng Wang, Hirofumi Kobayashi, Matthew N. McCarroll, Lachlan W. Whitehead, Reto P. Fiolka, Thomas B. Kornberg, Andrew G. York, Loic A. Royer