Synthetic biology offers a potential solution for enhanced carbon dioxide (CO₂) capture and conversion. This study demonstrates the design and construction of a novel CO₂-fixation pathway, the THETA cycle, composed of 17 enzymes from 9 organisms. Utilizing rational and machine learning-guided optimization, the cycle's yield was improved significantly, enabling the production of various biochemical building blocks directly from CO₂. The THETA cycle was modularized into three parts and successfully implemented in *Escherichia coli*, demonstrating a crucial step towards the development of complex, orthogonal CO₂-fixation pathways within living cells.
Publisher
Nature Catalysis
Published On
Dec 20, 2023
Authors
Shanshan Luo, Christoph Diehl, Hai He, Young Jun Bae, Melanie Klose, Peter Claus, Niña Socorro Cortina, Celia Alvarez Fernandez, Helena Schulz-Mirbach, Richard McLean, Adán Andrés Ramírez Rojas, Daniel Schindler, Nicole Paczia, Tobias J. Erb
Tags
synthetic biology
CO₂ fixation
biochemical production
THETA cycle
enzyme engineering
machine learning
Escherichia coli
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