Biology

Construction and modular implementation of the THETA cycle for synthetic CO₂ fixation

S. Luo, C. Diehl, et al.

Discover how a team of innovative researchers, including Shanshan Luo, Christoph Diehl, and Tobias J. Erb, has engineered a groundbreaking CO₂-fixation pathway known as the THETA cycle, capable of producing valuable biochemicals from carbon dioxide. This pioneering study not only showcases advancements in synthetic biology but also represents a significant stride towards sustainable solutions for carbon capture and utilization.

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Abstract

Synthetic biology offers a potential solution for enhanced carbon dioxide (CO₂) capture and conversion. This study demonstrates the design and construction of a novel CO₂-fixation pathway, the THETA cycle, composed of 17 enzymes from 9 organisms. Utilizing rational and machine learning-guided optimization, the cycle's yield was improved significantly, enabling the production of various biochemical building blocks directly from CO₂. The THETA cycle was modularized into three parts and successfully implemented in *Escherichia coli*, demonstrating a crucial step towards the development of complex, orthogonal CO₂-fixation pathways within living cells.

Publisher

Nature Catalysis

Published On

Dec 20, 2023

Authors

Shanshan Luo, Christoph Diehl, Hai He, Young Jun Bae, Melanie Klose, Peter Claus, Niña Socorro Cortina, Celia Alvarez Fernandez, Helena Schulz-Mirbach, Richard McLean, Adán Andrés Ramírez Rojas, Daniel Schindler, Nicole Paczia, Tobias J. Erb

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