Biosynthesized Silver Nanoparticle (AgNP) From *Pandanus odorifer* Leaf Extract Exhibits Anti-metastasis and Anti-biofilm Potentials

Cancer and associated secondary bacterial infections are leading causes of mortality due to a lack of effective drugs. This study synthesized silver nanoparticles (AgNPs) from an organic source and confirmed their anti-cancer and antimicrobial potentials. Microwave irradiation synthesized AgNPs using *Pandanus odorifer* leaf extract. AgNPs inhibited RBL cell migration (scratch assay). AgNPs exhibited MIC values of 4–16 µg/mL against Gram-positive and Gram-negative bacteria, demonstrating antimicrobial potential. Biofilm inhibition was observed at sub-MIC values. Violacein and alginate production were significantly reduced, suggesting anti-quorum sensing activity. Exopolysaccharide production and swarming motility were also significantly reduced. *In vivo* studies in mice confirmed tolerability, indicating clinical potential. The synthesized AgNPs exhibit both anti-metastasis and antimicrobial activities.

Afzal Hussain, Mohamed F. Alajmi, Meraj A. Khan, Syed A. Pervez, Faheem Ahmed, Samira Amir, Fohad M. Husain, Mohd S. Khan, Gouse M. Shaik, Iftekhar Hassan, Rais A. Khan, Md. Tabish Rehman