Biology

All-optical closed-loop voltage clamp for precise control of muscles and neurons in live animals

A. C. F. Bergs, J. F. Liewald, et al.

Discover the groundbreaking optogenetic voltage-clamp (OVC) technique developed by Amelie C. F. Bergs and colleagues, enabling precise control of excitable cells in live animals. This innovative method promotes high-throughput, contactless electrophysiology and offers true optogenetic control in behaving animals, providing unprecedented insights into cellular physiology.

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Abstract

This paper introduces an optogenetic voltage-clamp (OVC) technique for precise control of excitable cells in live animals. The OVC combines the voltage indicator QuasAr2 with the bidirectional optogenetic actuator BiPOLES, providing fast, closed-loop optical feedback. It successfully clamps voltage in *C. elegans* muscles and neurons, and rat hippocampal neurons, allowing for the measurement of optical I/V relationships and the detection of homeostatic changes in cellular physiology. The OVC facilitates high-throughput, contactless electrophysiology and enables true optogenetic control in behaving animals.

Publisher

Nature Communications

Published On

Apr 06, 2023

Authors

Amelie C. F. Bergs, Jana F. Liewald, Silvia Rodriguez-Rozada, Qiang Liu, Christin Wirt, Artur Bessel, Nadja Zeitzschel, Hilal Durmaz, Adrianna Nozownik, Holger Dill, Maëlle Jospin, Johannes Vierock, Cornelia I. Bargmann, Peter Hegemann, J. Simon Wiegert, Alexander Gottschalk

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