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Abstract
This paper introduces an optogenetic voltage-clamp (OVC) technique for precise control of excitable cells in live animals. The OVC combines the voltage indicator QuasAr2 with the bidirectional optogenetic actuator BiPOLES, providing fast, closed-loop optical feedback. It successfully clamps voltage in *C. elegans* muscles and neurons, and rat hippocampal neurons, allowing for the measurement of optical I/V relationships and the detection of homeostatic changes in cellular physiology. The OVC facilitates high-throughput, contactless electrophysiology and enables true optogenetic control in behaving animals.
Publisher
Nature Communications
Published On
Apr 06, 2023
Authors
Amelie C. F. Bergs, Jana F. Liewald, Silvia Rodriguez-Rozada, Qiang Liu, Christin Wirt, Artur Bessel, Nadja Zeitzschel, Hilal Durmaz, Adrianna Nozownik, Holger Dill, Maëlle Jospin, Johannes Vierock, Cornelia I. Bargmann, Peter Hegemann, J. Simon Wiegert, Alexander Gottschalk
Tags
optogenetics
voltage-clamp
excitable cells
high-throughput electrophysiology
cellular physiology
C. elegans
neuroscience

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