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Agrobacterium expressing a type III secretion system delivers Pseudomonas effectors into plant cells to enhance transformation

Agriculture

Agrobacterium expressing a type III secretion system delivers Pseudomonas effectors into plant cells to enhance transformation

V. Raman, C. M. Rojas, et al.

This groundbreaking research conducted by Vidhyavathi Raman, Clemencia M. Rojas, and their team engineered Agrobacterium tumefaciens to enhance plant transformation efficiency significantly. By incorporating a type III secretion system, they achieved remarkable increases in the transformation of wheat, alfalfa, and switchgrass, along with successful delivery of plant proteins, paving the way for advancements in agricultural biotechnology.... show more
Abstract
Agrobacterium-mediated plant transformation (AMT) underpins modern plant biotechnology, but many species/varieties are recalcitrant to Agrobacterium infection, likely due to elicited plant defense. The authors engineered Agrobacterium tumefaciens to express a type III secretion system (T3SS) from Pseudomonas syringae and to deliver individual P. syringae effectors (AvrPto, AvrPtoB, HopAO1) to suppress host defense. Using engineered Agrobacterium, AMT efficiency increased by approximately 250%–400% in wheat, alfalfa and switchgrass. They also show engineered A. tumefaciens expressing a T3SS can deliver a plant protein, histone H2A-1, to enhance AMT. This strategy benefits basic research and agricultural biotechnology for transient and stable transformation of recalcitrant plants and for non-transgenic protein delivery into plant cells.
Publisher
Nature Communications
Published On
May 11, 2022
Authors
Vidhyavathi Raman, Clemencia M. Rojas, Balaji Vasudevan, Kevin Dunning, Jaydeep Kolape, Sunhee Oh, Jianfei Yun, Lishan Yang, Guangming Li, Bikram D. Pant, Qingzhen Jiang, Kirankumar S. Mysore
DOI
https://doi.org/10.1038/s41467-022-30180-3
Tags
Agrobacterium
plant transformation
biotechnology
type III secretion system
wheat
alfalfa
switchgrass

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