Space Sciences

Surface tension enables induced pluripotent stem cell culture in commercially available hardware during spaceflight

M. Mozneb, M. Arzt, et al.

This groundbreaking research, conducted by a team of experts including Maedeh Mozneb and Peggy A. Whitson, explores the effects of microgravity on human induced pluripotent stem cells and their derivatives, providing a foundation for future biomanufacturing experiments in space.

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Playback language: English

Index

Abstract

Low Earth Orbit (LEO) offers a unique environment to study altered stem cell properties in microgravity. This study, conducted during Axiom Mission 2 (Ax-2) to the ISS, sent frozen human induced pluripotent stem cells (hiPSCs) and hiPSC-derived fibroblasts to space. Astronauts thawed and cultured these cells in commercially available 96-well plates. Surface tension within the wells prevented media loss, enabling hiPSC and fibroblast survival in suspension or on Matrigel. Successful transfection with RFP-expressing plasmids was also demonstrated. This research establishes a foundation for future biomanufacturing experiments in space.

Publisher

npj Microgravity

Published On

Oct 15, 2024

Authors

Maedeh Mozneb, Madelyn Arzt, Pinar Mesci, Dylan M. N. Martin, Stephany Pohlman, George Lawless, Shankini Doraisingam, Sultan Al Neyadi, Rayyanah Barnawi, Ali Al Qarni, Peggy A. Whitson, John Shoffner, Jana Stoudemire, Stefanie Countryman, Clive N. Svendsen, Arun Sharma

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